Products / Services

NEB offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification, qPCR and PCR. Our amplification technologies product portfolio includes a broad variety of both DNA and RNA polymerases, nucleotide mixes, kits, master mixes and more, featuring:

NEB scientists continue to improve its portfolio of restriction enzymes, as well as explore their utility in new technologies. As a result, NEB scientists continue to publish scientific papers and to be awarded grants in this area. With the industrys largest research and development group dedicated to restriction enzymes, we are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme, and the first to supply a true restriction enzyme master mix. In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance.

RNA molecules play a multitude of cellular roles in all kingdoms of life. RNA is an essential carrier of genetic information that can catalyze chemical reactions and influence gene expression.

In the last several years, our understanding of RNAs as regulatory molecules in the cell has dramatically changed. Many new classes of small and large non-coding RNAs with largely unexplored functions have been recently reported, ushering in a renaissance of RNA-focused research in biology.

SNAP- and CLIP-tag protein labeling systems enable the specific, covalent attachment of virtually any molecule to a protein of interest. There are two steps to using this system: cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. The SNAP-tag is a small protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein. SNAP-tag substrates are dyes, fluorophores, biotin, or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. CLIP-tag is a modified version of SNAP-tag, engineered to react with benzylcytosine rather than benzylguanine derivatives. When used in conjunction with SNAP-tag, CLIP-tag enables the orthogonal and complementary labeling of two proteins simultaneously in the same cells.

New England Biolabs has called upon its 35 years of expertise in enzymology to develop a suite of validated reagents for epigenetics research. This line of easy-to-use EpiMark kits simplifies DNA methylation and hydroxymethylation detection and analysis, as well as ChIP, histone and nucleosome analysis. Independently applicable, individual epigenetics reagents also complement the EpiMark kits. NEB's methylation- and hydroxymethylation- sensitive or dependent enzymes, DNA methyltransferases and DNA controls are all useful for mapping DNA modifications and methylating DNA at specific sites for gene expression studies. Our protein methyltransferases and recombinant histones perform efficiently in protein modification and characterization studies. Our range of modified and unmodified genomic DNAs can be used as controls for detection of DNA methylation. Our series of human DNA (cytosine-5) methyltransferase (DNMT) antibodies are ideally suited for Western blots and immunoprecipitation.

Proteome Analysis

Trypsin-digested BSA MS Standard (CAM-modified) (NEB #P8108) is a complex mixture of peptides that can be used to calibrate mass spectrometers.

Proteases

NEB offers several proteases for proteomics applications, including Trypsin (NEB #P8101), AspN (NEB #P8104), GluC (NEB #P8100), Furin (NEB #P8077) and Thermitase.

Protein Labeling

New England Biolabs provides the SNAP-tag, CLIP-tag and ACPMCP-tag protein labeling systems that facilitate the attachment of virtually any molecule to a protein of interest, and can be used for studying the function and localization of proteins in living and fixed cells, as well as protein immobilization.

Phage Display

The Ph.D. libraries from New England Biolabs have become the preeminent tools in this field, with hundreds of publications describing applications, including epitope mappingvaccine development, mapping protein-protein contacts and identification of peptide mimics of non-peptide ligands. Bioactive peptides, which can be used as cell-targeting or gene delivery agents, have been identified either by panning against purified receptors or against intact cells or tissue samples, both in vitro and in vivo. NEB offers displayed peptide libraries of 7 (NEB #E8100) and 12 (NEB #E8110) residues, as well as a disulfide-constrained 7-residue library (NEB #E8120).

Protein Kinases

The reversible addition of phosphate groups to proteins is important for the transmission of signals within eukaryotic cells and, as a result, protein phosphorylation and dephosphorylation regulate many diverse cellular processes. As the number of known protein kinases has increased at an ever-accelerating pace, it has become more challenging to determine which protein kinases interact with which substrates in the cell. The determination of consensus phosphorylation site motifs by amino acid sequence alignment of known substrates has proven useful in this pursuit. These motifs can be helpful for predicting phosphorylation sites for specific protein kinases within a potential protein substrate. NEB offers a line of several protein kinases to aid in this research.

Protein Phosphatases

Protein phosphorylation plays a role in the regulation of the function and activity of protein factors and enzymes. Protein phosphorylation can be found on tyrosine, histidine, serine and threonine residues. Analysis of the presence of such phosphorylation, and its attendant effects, is often aided by removal of the protein phosphate groups by phosphatases. NEB offers several protein phosphatases that remove phosphate groups from different subsets of these residues.

Protein Standards

New England Biolabs offers a selection of highly pure protein standards. Sizes range from 10 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. We offer a blue prestained protein standard, as well as a colored prestained protein standard with multi-colored bands for easy identification. Standards are provided pre-mixed with loading buffer and reducing agent.

New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are not hydrolyzed by some exoglycosidases, we use only fluorescently-labeled oligosaccharides to screen for contaminating glycosidases.

Cloning Strains

For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.

Yeast

NEB offers chemically competent Kluyveromyces lactis cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.

E. coli for Protein Expression

NEB offers several strains with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies

Monarch nucleic acid purification kits provide fast and reliable purification of high quality DNA from bacterial cultures, agarose gels, and enzymatic reactions using best-in-class silica column technology. Monarchs unique column design eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification. Designed with sustainability in mind, Monarch kits use less plastic and are packaged with responsibly sourced materials. To view performance data for Monarch Nucleic Acid Purification Kits, use the links below:

Advances in molecular biology require constant innovation and forward progress in the tools and techniques at the core of the field. New England Biolabs is committed to providing a wide variety of essential reagents that serve to advance the frontier of DNA-based manipulations.

NEBs enzymology expertise sets it apart from competitors, allowing us to produce enzymes for molecular biology that deliver; our highly pure enzymes, over 250 of which are recombinant, offer exceptional performance and value.